mouse anti tmem16a  (Proteintech)

Journal: JCI Insight

Article Title: Active enterohepatic cycling is not required for the choleretic actions of 24-norUrsodeoxycholic acid in mice

doi: 10.1172/jci.insight.149360

Figure Lengend Snippet: ( A ) Representative whole-cell currents in MLC cells transfected with nontargeting siRNA or TMEM16A siRNA measured under basal conditions or during exposure to norUDCA (250 μM). Currents measured at –100 mV (black) or +100 mV (red), representing I Cl – are shown. Compound exposure is indicated by the black bar. A voltage-step protocol from a holding potential of –40 mV, with 450 ms steps from –100 to +100 mV in 20 mV increments. Currents demonstrated time-dependent activation at membrane potentials +100 mV. The I-V plot was generated from these protocols during basal (black) and norUDCA-stimulated (red) conditions. ( B ) Cumulative data demonstrating maximum increase in current density (pA/pF) in response to norUDCA in the absence or presence of TMEM16A siRNA silencing; n = 5–6 cells per group. Median values (line), interquartile range (boxes), and minimum to maximum values (whiskers) are shown. The data were evaluated for statistically significant differences using an ordinary 2-way ANOVA with a Tukey’s multiple-comparison test.

Article Snippet: The degree of TMEM16A silencing was evaluated by Western blot analysis using anti–mouse TMEM16A antibody (1:1000, Alomone Laboratories, ACL-011) ( ).

Techniques: Transfection, Activation Assay, Membrane, Generated, Comparison

Journal: JCI Insight

Article Title: Active enterohepatic cycling is not required for the choleretic actions of 24-norUrsodeoxycholic acid in mice

doi: 10.1172/jci.insight.149360

Figure Lengend Snippet: ( A ) Representative whole-cell currents in MLC cells measured under basal conditions and during exposure to norUDCA (250 μM) following preincubation with vehicle (top), TMEM16A inhibitor (10 μM A01; middle), or ASBTi (100 nM SC-435; bottom). Currents measured at –100 mV (black) or +100 mV (red) representing I Cl – are shown. Compound exposure is indicated by the black bar. The I-V plot was generated from these protocols during basal (black) and norUDCA-stimulated (red) conditions. ( B ) Cumulative data demonstrating maximum increase in current density (pA/pF) in response to norUDCA in the absence or presence of TMEM16A inhibitor or ASBTi; n = 5–35 cells per group. The data were evaluated for statistically significant differences using an ordinary 1-way ANOVA with a Tukey’s multiple-comparison test. Values with distinct superscript lowercase letters are significantly different ( P < 0.05).

Article Snippet: The degree of TMEM16A silencing was evaluated by Western blot analysis using anti–mouse TMEM16A antibody (1:1000, Alomone Laboratories, ACL-011) ( ).

Techniques: Generated, Comparison

Journal: Therapeutics and Clinical Risk Management

Article Title: Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature

doi: 10.2147/TCRM.S167248

Figure Lengend Snippet: Ileal GISTs and immunohistochemistry. Notes: A 55-year-old male with an ileal GISTs presented with melena. ( A1 ) Axial plain CT image does not reveal the primary tumor due to polymorphous intestine. ( A2 ) Axial contrast-enhanced CT image shows a 1.3×1.8 cm 2 tumor (arrow) with marked enhancement in the arterial phase. ( A3 ) DBE shows the intraluminal component of the tumor with engorged vessels. ( A4 ) Gross appearance of the operative specimen shows the extraluminal component of the tumor (arrow). ( B1 ) Histopathology shows the tumor cells are composed of spindle cells with a low mitotic count (≤5 mitoses/50 HPFs; HE staining). Tumor cells are strongly positive for CD117 ( B2 ), CD34 ( B3 ), and DOG1 ( B4 ). Tumor cells are negative for SMA ( C1 ), which is positive in vascular wall. S-100 ( C2 ) and Desmin ( C3 ) are negative in tumor cells. Ki-67 ( C4 ) is positive in some of the tumor nuclei (global index 2%). (Original magnification: B1 ×100; B2 – C4 ×200). Abbreviations: GISTs, gastrointestinal stromal tumors; CT, computed tomography; DBE, double-balloon enteroscopy; HPFs, high-power fields; HE, hematoxylin and eosin; SMA, smooth muscle actin.

Article Snippet: The routine diagnostic immunohistochemistry technique used a panel of seven primary antibodies purchased from Boster Biological Technology (Wuhan, China), which included a mouse anti-CD117 monoclonal antibody used at 1:100 dilution, a rabbit anti-CD34 polyclonal antibody used at 1:200 dilution, a mouse anti-DOG1 monoclonal antibody used at 1:200 dilution, a mouse anti-SMA monoclonal antibody used at 1:150 dilution, a mouse anti-S100 monoclonal antibody used at 1:50 dilution, a mouse anti-desmin monoclonal antibody used at 1:100 dilution, and a rabbit anti-Ki-67 monoclonal antibody used at 1:100 dilution.

Techniques: Immunohistochemistry, Histopathology, Staining, Computed Tomography